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murine epidermal growth factor (megf  (Millipore)


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    Structured Review

    Millipore murine epidermal growth factor (megf
    Murine Epidermal Growth Factor (Megf, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+epidermal+growth+factor+(megf/murine+epidermal+growth+factor/pmc11126719-182-75-80
    Average 90 stars, based on 1 article reviews
    murine epidermal growth factor (megf - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Combined Systemic Drug Treatment with Proton Therapy: Investigations on Patient-Derived Organoids.
    Article Snippet: .. PDOs were embedded in GFR Matrigel (Corning, Corning, NY, USA) and cultured in PDAC culture media based on DMEM/F12 (Gibco, Waltham, MA, USA) supplemented with Pen/Strep (1%, Gibco), GlutaMAX (1%, Gibco), HEPES (1%, Gibco), Wnt3a (100 ng/mL, R&D Systems), R-Spondin (250 ng/mL, PeproTech, Hamburg, Germany), Noggin (100 ng/mL, PeproTech), B27 (1×, Gibco), Nicotinamid (10 mM, SigmaAldrich, St. Louis, MO, USA), Gastrin (10 nM, Sigma-Aldrich), N-actyl-L-cysteine (1.25 mM, Sigma-Aldrich), Primocin (100 μg/mL, InvivoGen, San Diego, CA, USA), mEGF (50 ng/mL, Sigma-Aldrich), A-83-01 (0.5 μM, Tocris, Bristol, UK), recombinant human fibroblast growth factor 10 (hFGF-10, 100 ng/mL, PeproTech), murine recombinant epidermal growth factor (mEGF, 50 ng/mL, Sigma-Aldrich) and N2 (1×, Gibco). ..

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. KB2P3.4 ( Trp53 -/- ;Brca2 -/- ), KB2P1.21 ( Trp53 -/- ;Brca2 -/- ), and KB1P-G3 ( Trp53 -/- ;Brca1 -/- ) cells and all their derivative cell lines were grown at 37 °C in 3% O 2 and cultured in Dulbecco’s modified Eagle medium Nutrient mixture F-12 (DMEM/F12; Gibco) supplemented with 10% Fetal Bovine Serum (FBS), pen/strep solution (50 U/ml), 5 ng/ml cholera toxin (Sigma Aldrich), insulin (5 μg/ml, Sigma Aldrich) and 5 ng/ml murine Epidermal Growth Factor (mEGF, Sigma Aldrich). .. ORG-KB2P17S.1 organoids were grown at 37 °C in normal oxygen embedded in Cultrex Reduced Growth Factor Basement Membrane Extract Type 2 (BME, Trevigen), seeded on 24-well suspension plates (Greiner Bio-One) and cultured in complete mouse mammary gland organoid medium: AsDMEM/F12 supplemented with 1 M HEPES (Sigma), GlutaMAX (Invitrogen), pen/strep (Gibco), B27 (Gibco), 125μM N-acetyl-L-cysteine (Sigma), 50 ng/ml murine epidermal growth factor (mEGF, Invitrogen).

    Article Title: Combined Systemic Drug Treatment with Proton Therapy: Investigations on Patient-Derived Organoids
    Article Snippet: .. PDOs were embedded in GFR Matrigel (Corning, Corning, NY, USA) and cultured in PDAC culture media based on DMEM/F12 (Gibco, Waltham, MA, USA) supplemented with Pen/Strep (1%, Gibco), GlutaMAX (1%, Gibco), HEPES (1%, Gibco), Wnt3a (100 ng/mL, R&D Systems), R-Spondin (250 ng/mL, PeproTech, Hamburg, Germany), Noggin (100 ng/mL, PeproTech), B27 (1×, Gibco), Nicotinamid (10 mM, Sigma-Aldrich, St. Louis, MO, USA), Gastrin (10 nM, Sigma-Aldrich), N-actyl-L-cysteine (1.25 mM, Sigma-Aldrich), Primocin (100 μg/mL, InvivoGen, San Diego, CA, USA), mEGF (50 ng/mL, Sigma-Aldrich), A-83-01 (0.5 μM, Tocris, Bristol, UK), recombinant human fibroblast growth factor 10 (hFGF-10, 100 ng/mL, PeproTech), murine recombinant epidermal growth factor (mEGF, 50 ng/mL, Sigma-Aldrich) and N2 (1×, Gibco). ..

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. All mouse-derived cells were grown at 37oC in 3% O 2 and cultured in Dulbecco’s modified Eagle medium Nutrient mixture F-12 (DMEM/F12; Gibco) supplemented with 10% Fetal Bovine Serum (FBS), pen/strep solution (50 U/ml), 5 ng/ml cholera toxin (Sigma Aldrich), insulin (5 μg/ml, Sigma Aldrich) and 5 ng/ml murine Epidermal Growth Factor (mEGF, Sigma Aldrich). .. ORG-KB2P17S.1 organoids were grown at 37oC in normal oxygen embedded in Cultrex Reduced Growth Factor Basement Membrane Extract Type 2 (BME, Trevigen), seeded on 24-well suspension plates (Greiner Bio-One) and cultured in complete mouse mammary gland organoid medium: AsDMEM/F12 supplemented with 1M HEPES (Sigma), GlutaMAX (Invitrogen), pen/strep (Gibco), B27 (Gibco), 125μM N-acetyl-L-cysteine (Sigma), 50 ng/ml murine epidermal growth factor (mEGF, Invitrogen).

    Recombinant:

    Article Title: Combined Systemic Drug Treatment with Proton Therapy: Investigations on Patient-Derived Organoids.
    Article Snippet: .. PDOs were embedded in GFR Matrigel (Corning, Corning, NY, USA) and cultured in PDAC culture media based on DMEM/F12 (Gibco, Waltham, MA, USA) supplemented with Pen/Strep (1%, Gibco), GlutaMAX (1%, Gibco), HEPES (1%, Gibco), Wnt3a (100 ng/mL, R&D Systems), R-Spondin (250 ng/mL, PeproTech, Hamburg, Germany), Noggin (100 ng/mL, PeproTech), B27 (1×, Gibco), Nicotinamid (10 mM, SigmaAldrich, St. Louis, MO, USA), Gastrin (10 nM, Sigma-Aldrich), N-actyl-L-cysteine (1.25 mM, Sigma-Aldrich), Primocin (100 μg/mL, InvivoGen, San Diego, CA, USA), mEGF (50 ng/mL, Sigma-Aldrich), A-83-01 (0.5 μM, Tocris, Bristol, UK), recombinant human fibroblast growth factor 10 (hFGF-10, 100 ng/mL, PeproTech), murine recombinant epidermal growth factor (mEGF, 50 ng/mL, Sigma-Aldrich) and N2 (1×, Gibco). ..

    Article Title: Combined Systemic Drug Treatment with Proton Therapy: Investigations on Patient-Derived Organoids
    Article Snippet: .. PDOs were embedded in GFR Matrigel (Corning, Corning, NY, USA) and cultured in PDAC culture media based on DMEM/F12 (Gibco, Waltham, MA, USA) supplemented with Pen/Strep (1%, Gibco), GlutaMAX (1%, Gibco), HEPES (1%, Gibco), Wnt3a (100 ng/mL, R&D Systems), R-Spondin (250 ng/mL, PeproTech, Hamburg, Germany), Noggin (100 ng/mL, PeproTech), B27 (1×, Gibco), Nicotinamid (10 mM, Sigma-Aldrich, St. Louis, MO, USA), Gastrin (10 nM, Sigma-Aldrich), N-actyl-L-cysteine (1.25 mM, Sigma-Aldrich), Primocin (100 μg/mL, InvivoGen, San Diego, CA, USA), mEGF (50 ng/mL, Sigma-Aldrich), A-83-01 (0.5 μM, Tocris, Bristol, UK), recombinant human fibroblast growth factor 10 (hFGF-10, 100 ng/mL, PeproTech), murine recombinant epidermal growth factor (mEGF, 50 ng/mL, Sigma-Aldrich) and N2 (1×, Gibco). ..

    Modification:

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. KB2P3.4 ( Trp53 -/- ;Brca2 -/- ), KB2P1.21 ( Trp53 -/- ;Brca2 -/- ), and KB1P-G3 ( Trp53 -/- ;Brca1 -/- ) cells and all their derivative cell lines were grown at 37 °C in 3% O 2 and cultured in Dulbecco’s modified Eagle medium Nutrient mixture F-12 (DMEM/F12; Gibco) supplemented with 10% Fetal Bovine Serum (FBS), pen/strep solution (50 U/ml), 5 ng/ml cholera toxin (Sigma Aldrich), insulin (5 μg/ml, Sigma Aldrich) and 5 ng/ml murine Epidermal Growth Factor (mEGF, Sigma Aldrich). .. ORG-KB2P17S.1 organoids were grown at 37 °C in normal oxygen embedded in Cultrex Reduced Growth Factor Basement Membrane Extract Type 2 (BME, Trevigen), seeded on 24-well suspension plates (Greiner Bio-One) and cultured in complete mouse mammary gland organoid medium: AsDMEM/F12 supplemented with 1 M HEPES (Sigma), GlutaMAX (Invitrogen), pen/strep (Gibco), B27 (Gibco), 125μM N-acetyl-L-cysteine (Sigma), 50 ng/ml murine epidermal growth factor (mEGF, Invitrogen).

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. All mouse-derived cells were grown at 37oC in 3% O 2 and cultured in Dulbecco’s modified Eagle medium Nutrient mixture F-12 (DMEM/F12; Gibco) supplemented with 10% Fetal Bovine Serum (FBS), pen/strep solution (50 U/ml), 5 ng/ml cholera toxin (Sigma Aldrich), insulin (5 μg/ml, Sigma Aldrich) and 5 ng/ml murine Epidermal Growth Factor (mEGF, Sigma Aldrich). .. ORG-KB2P17S.1 organoids were grown at 37oC in normal oxygen embedded in Cultrex Reduced Growth Factor Basement Membrane Extract Type 2 (BME, Trevigen), seeded on 24-well suspension plates (Greiner Bio-One) and cultured in complete mouse mammary gland organoid medium: AsDMEM/F12 supplemented with 1M HEPES (Sigma), GlutaMAX (Invitrogen), pen/strep (Gibco), B27 (Gibco), 125μM N-acetyl-L-cysteine (Sigma), 50 ng/ml murine epidermal growth factor (mEGF, Invitrogen).

    other:

    Article Title: Single cell transcriptomics reveals molecular subtype and functional heterogeneity in models of breast cancer
    Article Snippet: Comma-Dβ cells were maintained in DMEM/F12 media (Gibco®), supplemented with 2% FBS (Thermo-Scientific), 10 mM HEPES (Gibco ®), 5 ml penicillin/streptomycin (Gibco ®), 0.25% insulin (Novo Nordisk) and 5 ng ml −1 murine epidermal growth factor (mEGF) (Sigma).



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    Image Search Results


    Journal: Cells

    Article Title: Isolation of Myenteric and Submucosal Plexus from Mouse Gastrointestinal Tract and Subsequent Co-Culture with Small Intestinal Organoids

    doi: 10.3390/cells13100815

    Figure Lengend Snippet: Krebs Solution.

    Article Snippet: 1 mg of GibcoTM murine Epidermal Growth Factor (mEGF) Recombinant Protein (MG8043, fisherscientific). Prepare 500 μg/mL murine in 0.1% Bovine Serum Albumin (BSA) in Phosphate-buffered saline (PBS) to create a 10,000X stock solution. Store at −80 °C..

    Techniques:

    Small intestinal organoid culture reagents.

    Journal: Cells

    Article Title: Isolation of Myenteric and Submucosal Plexus from Mouse Gastrointestinal Tract and Subsequent Co-Culture with Small Intestinal Organoids

    doi: 10.3390/cells13100815

    Figure Lengend Snippet: Small intestinal organoid culture reagents.

    Article Snippet: 1 mg of GibcoTM murine Epidermal Growth Factor (mEGF) Recombinant Protein (MG8043, fisherscientific). Prepare 500 μg/mL murine in 0.1% Bovine Serum Albumin (BSA) in Phosphate-buffered saline (PBS) to create a 10,000X stock solution. Store at −80 °C..

    Techniques: Membrane, Recombinant, Saline, Transfection, Expressing

    Preparation of 500 μg/mL  murine Epidermal Growth Factor (mEGF) Recombinant Protein  in 0.1% BSA in PBS 1X to create a 10,000× stock solution.

    Journal: Cells

    Article Title: Isolation of Myenteric and Submucosal Plexus from Mouse Gastrointestinal Tract and Subsequent Co-Culture with Small Intestinal Organoids

    doi: 10.3390/cells13100815

    Figure Lengend Snippet: Preparation of 500 μg/mL murine Epidermal Growth Factor (mEGF) Recombinant Protein in 0.1% BSA in PBS 1X to create a 10,000× stock solution.

    Article Snippet: 1 mg of GibcoTM murine Epidermal Growth Factor (mEGF) Recombinant Protein (MG8043, fisherscientific). Prepare 500 μg/mL murine in 0.1% Bovine Serum Albumin (BSA) in Phosphate-buffered saline (PBS) to create a 10,000X stock solution. Store at −80 °C..

    Techniques: Recombinant, Saline

    Small intestinal organoid culture reagents.

    Journal: Cells

    Article Title: Isolation of Myenteric and Submucosal Plexus from Mouse Gastrointestinal Tract and Subsequent Co-Culture with Small Intestinal Organoids

    doi: 10.3390/cells13100815

    Figure Lengend Snippet: Small intestinal organoid culture reagents.

    Article Snippet: 1 mg of Gibco™ murine Epidermal Growth Factor (mEGF) Recombinant Protein (MG8043, fisherscientific). Prepare 500 μg/mL murine in 0.1% Bovine Serum Albumin (BSA) in Phosphate-buffered saline (PBS) to create a 10,000X stock solution. Store at −80 °C..

    Techniques: Membrane, Recombinant, Saline, Transfection, Expressing

    Preparation of 500 μg/mL  murine Epidermal Growth Factor (mEGF) Recombinant Protein  in 0.1% BSA in PBS 1X to create a 10,000× stock solution.

    Journal: Cells

    Article Title: Isolation of Myenteric and Submucosal Plexus from Mouse Gastrointestinal Tract and Subsequent Co-Culture with Small Intestinal Organoids

    doi: 10.3390/cells13100815

    Figure Lengend Snippet: Preparation of 500 μg/mL murine Epidermal Growth Factor (mEGF) Recombinant Protein in 0.1% BSA in PBS 1X to create a 10,000× stock solution.

    Article Snippet: 1 mg of Gibco™ murine Epidermal Growth Factor (mEGF) Recombinant Protein (MG8043, fisherscientific). Prepare 500 μg/mL murine in 0.1% Bovine Serum Albumin (BSA) in Phosphate-buffered saline (PBS) to create a 10,000X stock solution. Store at −80 °C..

    Techniques: Recombinant, Saline